Laboratory of Structural Proteins of Eukaryotic Chromosomes (SPEC)

People involved:
 
Head:
Assoc. prof. Jan Palecek, Dr.

 

Description:

The SMC (structure maintenance of chromosome) complexes are conserved from bacteria to humans. They are key components of higher-order chromatin structures and play important roles in genome stability. Three SMC complexes are present in most eukaryotic cells: cohesin (SMC1/3), condensin (SMC2/4) and SMC5/6 complex. Cohesin can embrace two sister chromatids and is essential for proper chromosome segregation. Condensin is critical for mitotic chromosome condensation. The SMC5/6 complex is involved in the homologous recombination-based DNA repair, in replication fork stability and processing, and in cohesin regulation.
     In our lab, we study assembly and functions of the SMC5/6 subunits at molecular level. We use combination of genetic and biochemical methods to get deep insight into SMC5/6 features. Recently, we discovered several important features of the Nse3 (non-SMC element) KITE subunit: its role in structural organization of the SMC5/6 complex, its DNA-binding ability, its evolution from bacteria to novel mammalian (MAGE) protein family. In addition, we participated in characterization of new human chromosome breakage syndrome associated with Nse3 mutations.
 

Laboratory web:  

ncbr.chemi.muni.cz/SPEC/
 

 
Members of the Laboratory of Structural Proteins of Eukaryotic Chromosomes

Figure: Members of the Laboratory of Structural Proteins of Eukaryotic Chromosomes.


 

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