Studies of Proton-transfer Processes and Complexations of Purine Derivatives
Doc. RNDr. Radek Marek, Ph.D.
Mgr. Jaromír Toušek, Dr.
Prof. RNDr. Vladimír Sklenář, DrSc.
Diploma, Ph.D. and postdoctoral fellows:
Mgr. Kateřina Maliňáková
Mgr. Sečkařová Pavlína
Purines play a central role in all living systems. They are not only constituens of nucleic
acids but also interact with enzymes and other proteins. Their biological
and pharmacological effects are connected with the ability to form
supramolecular complexes with biological targets. The hydrogen bonds play
crucial role in such supramolecular interactions.
15N NMR spectroscopy has been found essential to furnish information about the
protonation site(s) on the purine ring, the proton-transfer equilibria and the
hydrogen-bond formation. 15N NMR chemical shifts and
1H-15N coupling constants are obtained by using
inverse-detected multiple bond chemical shift correlation techniques at
15N natural-abundance level. Low temperature measurements of
13C and 15N chemical shifts and 3JH,C a 2JH,N coupling constants are used
for determining the proton-transfer proceses and interactions of purines with
metal ions. The solid-state NMR, x-ray diffraction, and quantum chemical
calculations are also extensively used in the structural
Diploma and Ph.D. theses offered:
List of selected publications:
Marek, R.; Lyčka, A. 2002. 15N NMR spectroscopy in structural
analysis. Curr. Org. Chem. 6: 35 - 66.
Marek, R.; Brus, J.; Toušek, J.; Kovács, J.; and Hocková, D. 2002.
N7- and N9-substituted purine derivatives: a
15N NMR study. Magn. Reson. Chem.40: 353 - 360.
Jiří Brus, Institute of Macromolecular Chemistry AS CR, Prague, Czech Republic
Michal Hocek, Institute of Organic Chemistry and Biochemistry AS CR, Prague, Czech Republic
Zdeněk Trávníček, Palacký University, Olomouc, Czech Republic
Lajos Kovács, University of Szeged, Hungary
Erkki Kolehmainen, University of Jyväskylä, Finland
Emmanuel Mikros, University of Athens, Greece