Novel Experimental Techniques in Nuclear Magnetic Resonance of Biomolecules
A general goal of NMR studies of biomolecules is to obtain as accurate as possible
data that can characterize the structure and/or dynamics of the studied system.
A necessary first step in any NMR study is the resonance assignment. For this
purpose, the methods that rely on through-bond interactions are preferable.
Methods to allow complete resonance assignment of both proteins and nucleic
acids have been developed by now. Today, the task is to
sensitivity and resolution of the methods to allow application to
bimolecular systems of increasing complexity.
Example of a pulse scheme for a two-dimensional NMR experiment. The scheme shown is for the HCNC experiment proposed for establishing connectivities between H8 and C4 in the purine bases and between H6 and C2 in the pyrimidine bases of nucleic acids (Fiala et al. J. Biomol. NMR 2004 29: 477-490).