Research group RNA Quality Control
Assoc. Prof. Mgr. Štěpánka Vaňáčová, Ph.D.
RNAs are involved in many key cellular processes, such as DNA, RNA and protein synthesis. If changed, RNAs can loose their normal activity or lead to production of defective molecules. The consequences may be detrimental for the cell and lead to disease at the organism level. To prevent an accumulation of defective RNA molecules, eukaryotes have evolved sophisticated surveillance mechanisms to tightly control the quality and abundance of RNAs. Recent findings in yeast have revealed the existence of quality control pathway in which the TRAMP poly(A) polymerase complex together with the exosome complex target a broad range of aberrant RNAs for degradation.
Our aim is to investigate the molecular basis for the RNA substrate selection by the TRAMP complex. One possibility is that specific, still undiscovered, features common to misfolded RNAs are recognized. Alternatively, the RNA surveillance proteins interact with general RNA structures, whereas correctly folded RNAs are sequestered by specific RNA-binding proteins and thus protected from degradation. We use combination of biochemical, genetic, and structural methods to unravel the molecular mechanism of the TRAMP-mediated RNA surveillance.
- Mechanisms of nuclear RNA surveillance and degradation
- Mechanisms and function of noncanonical polyadenylation
- RNA uridylation and nucleotide modification
- Investigation of the role of RNA in development and human diseases
- Biochemical and structural characterization of RNA in eukaryotic cells
Members of the laboratory also participate in research group
RNA Quality Control
Figure: The RNA group, December 2011.
Design © oliver 2007