Prof. RNDr. Vladimír Sklenář, DrSc.



Education, Scientific and Pedagogical Carrier

Faculty of Science, Masaryk University, Brno, Chemistry, 1974.
RNDr degree: Department of Theoretical and Physical Chemistry
Faculty of Science, Masaryk University, Brno, 1975.
CSc. degree (equivalent to PhD.), Technical University, Brno, 1984.
Associate Professor, Faculty of Science, Masaryk University, Brno, 1992
DrSc. degree, Institute of Chemical Technology, Prague, 1996
Professor of Physical Chemistry, Faculty of Science, Masaryk University, Brno, 1997

Employment and Scientific Sojourns

Professional Interests

NMR methods, development of multi-dimensional FT NMR techniques, spin system dynamics, theoretical description of spin system behaviour, gradient-enhanced NMR spectroscopy, multiple-resonance techniques, Ab Initio calculations of NMR parameters, NMR-based structural studies of nucleic acids and proteins.





LIST OF RECENT PAPERS - (long list)

 

•    Pavliková, N. Bláhová, L.,  Klán, P.,  Bathula, S.R.,  Sklenář, V.,  Giesy, J. P., and Bláha, L.,
Chemosphere  2012,  86, 65–69: Enantioselective effects of alpha-hexachlorocyclohexane (HCH) isomers on androgen receptor activity in vitro

•    Fiala, R., Špačková, N., Foldynová-Trantírková, S., Šponer, J., Sklenář, V., Trantírek, L., J. Amer.Chem. Soc. 2011, 133, 13790–13793:
NMR cross­correlated relaxation rates reveal ion coordination sites in DNA.

•    Pekárová, B.,  Klumpler, T., Třísková, O., Horák, J., Jansen, S., Dopitová, R., Papoušková, V., Nejedlá, E., Sklenář, V., Marek, J., Žídek, L. , Hejátko, J., Janda, L., Plant J. 2011,  67, 827–839:
Structure and binding specificity of the receiver domain of sensor histidine kinase CKI1 from Arabidopsis thaliana

•    Nováček, J., Zawadska-Kazimierczuk, A., Motáčková, V., Žídek, L., Koźmiński, W., and Sklenář, V., J. Biomol. NMR. 2011, 50, 1–11:
5D 13C-detected NMR experiments for backbone assignment of unstructured proteins with a very low signal dispersion

•    Motáčková, V., Nováček, J, Zawadska-Kazimierczuk, A., Kazimierczuk, K., Žídek, L., Šanderová, H., Krásný, L.,  Koźmiński, W., and Sklenář, V., J. Biomol. NMR 2010,  48, 169–177:
Strategy for complete NMR assignment of disordered proteins with highly repetitive sequences based on resolution-enhanced 5D experiments

•    Přecechtělová, J., Novák, P,  Munzarová, M.L., M. Kaupp, and Sklenář, V., J. Amer. Chem. Soc. 2010, 132, 17139–17148:
31P Chemical Shift and Chemical Shift Anisotropy Tensors in DNA: DFT and MD Study

•    Kovalová, A., Ledvina, M., Šaman, D., Zyka, D., Kubíčková, M., Žídek, L., Sklenář, V., Pompach, P., Kavan, D., Bílý, J., Vaněk, O.,
Kubínková, Z., Libigerová, M., Ivanová, L, Antolíková, M., Mrázek, H., Rozbeský, D., Hofbauerová, K., Křen, V., and Bezouška, K.: J. Med. Chem. 2010, 53, 4050-4065:
Synthetic N-acetyl-D-glucosamine based fully branched tetrasaccharide, a mimetic of the endogenous ligand for CD69, activates CD69+ killer lymphocytes upon dimerization via a hydrophilic flexible linker

•    Motáčková, V.,  Šanderová, H., žídek, L., Nováček, J., Padrta, P., Švenková, A., Korelusová, J., Jonák, J., Krásný L. and Sklenář, V.: PROTEINS: Structure, Function, and Bioinformatics 2010,  78, 1807-1810:
 Solution Structure of the N-terminal domain of Bacillus subtilis  subunit of RNA polymerze and its classification based on structural homologues.

•    Kavan, D., Kubíčková, M.,  Bílý, J., Vaněk, O., Hofbauerová, K., Mrázek,  H., Rozbeský, D.,  Bojarová, P., Křen, V., Žídek, L., Sklenář, V., and Bezouška, K.: Biochemistry 2010, 49, 4060–4067:
Cooperativity between subunits is essential for high affinity binding of N-acetylhexosamines to dimeric soluble and dimeric cellular forms of human CD69.

•    Novák, P., Žídek, L.,  Motáčková, V., Padrta, P., Švenková, a., Nuzillard, J-M., Krásný, L., Sklenář, V.: J. Biomol. NMR 2010, 46, 191-197:
S3EPY: a Sparky extension for determination of small scalar couplings from spin-state-selective excitation NMR experiments.

•    Motáčková, V.,  Kubíčková, M., Kožíšek, M., Grantz Šašková, Švec, M., Žídek, L., and Sklenář, V., Biomol. NMR  Assign. 2009, 3, 261-264:
Backbone 1H, 13C, and 15N NMR assignment for the inactive form of the retroviral protease of the murine intracisternal A-type particle, inMIA-14 PR.

•    Matejková, M., Žídková, J., Žídek, L., Wimmerová, M., Chmelík, J., and Sklenář, V., J. Agr. Food Chem. 2009, 57, 8444-8452:
Investigation of Thermal Denaturation of Barley Non-Specific Lipid Transfer Protein 1 (ns-LTP1b) by Nuclear Magnetic Resonance and Differential Scanning Calorimetry.

•    Macek, P., Chmelík, J., Křížová, I., Kadeřávek, P. Žídek, L., Wildová, M.,  Hadravová, R.,  Chaloupková, R.,  Pichová, I.,  Ruml, T., Rumlová, M., Sklenář, V.: J. Mol. Biol. 2009, 392, 100-114:
NMR structure of the N-terminal domain of capsid protein from the Mason-Pfizer monkey virus

•    Vaněk, O., Nálezková, M., Kavan, D. Borovičková, Pompach, P., Novák, P., Kumar, V., Hudeček, J.,  Brynda, J.,  Kolenko, P.,
Dohnálek, J., Kadeřávek, Chmelík, J., Gorčík, L., Žídek, L., Sklenář, V. and Bezouška, K., FEBS Journal 2008, 275, 5589–5606.:
Soluble recombinant human CD69 receptors optimized to have an exceptional physical and chemical stability are effective in animal tumor therapies.

•    Macek, P., Žídek, L., Rumlová, M., Pichová, I., and Sklenář, V.: Biomol. NMR Assign. 2008, 2, 43-45 :
1H, 13C, and 15N Resonance Assignment of the N-terminal Domain of Mason-Pfizer Monkey Virus Capsid Protein, CA1-140.

•    Přecechtělová, J., Padrta, P,  Munzarová, M.L., and Sklenář, V.: J. Phys. Chem B  2008, 112, 3470-3478:
31P Chemical Shift Tensors for Canonical and Non-Canonical Nucleic Acid Conformations: DFT Calculations and NMR Implications.





National Centre for Biomolecular Research
NMR Laboratory
Faculty of Science, Masaryk University
Kotlarska 2, CZ-611 37 Brno
tel.:+420 - 549 497 022
fax :+420 - 549 492 556
e-mail: sklenar@chemi.muni.cz

last modified on 10.04.2008