Structural biology

PhD position OPEN !

We seek for a highly motivated candidate to join our lab.

Postdoc position OPEN !


 

Interdisciplinary projects

Our projects are supported by a strong biological and biochemical foundations. To broaden experience, students are stimulated to  perform a mixture of biochemical/biological experiments involving bench work as well as NMR experiments and structural calculations.

The Stefl lab is affiliated with the National Centre of Biomolecular Research at Central European Institute of Technology -- Masaryk University. We use nuclear magnetic resonance (NMR) spectroscopy to study interactions between proteins and RNAs with the aim to gain structural insights into the assembly and function of RNA processing and degradation machineries. You can find out more about our research and facilities on this site.

Our lab is located on the first and third floor of Building A4 in the University Campus Bohunice (see Contact page).

Facilities

We have access to the Josef Dadok National NMR Centre which equipped with 950/850/700/700/600/500 MHz Bruker spectometers. Most of them are equipped with four radiofrequency channels and a tripple resonance TCI (1H, 13C, 15N) cryoprobe with proton and carbon coils and preemplifiers operating at low temperature for increased sensitivity. Our laboratory offers full routine molecular biology (cloning/mutagenesis/PCR), protein expression and purification (FPLC, HPLC) and characterization (fluoresence spectroscopy). Isothermal titration calorimetry and surface plasmon resonance are offered within the Institute. Computing resources are extensive and feature an integrated Mac OS X UNIX front-end coupled to a dedicated distributed linux cluster backend for computations and structure calculations.