NCBR & SB Seminar series: Miroslav Krepl

Dynamic Recognition of Nucleic Acids by Proteins and the Role of Partial-disorder

Biomolecular dynamics help elucidate complex intramolecular interfaces, such as protein-RNA and protein-DNA complexes, influencing binding affinities, specificities, and formation rates. These interfaces involve dynamic conformational sub-states rather than fixed geometries, enabling dynamic recognition. This mechanism aids highly specific RNA recognition without compromising biological function and is shaped by molecular evolution to optimize binding and kinetics. Dynamic recognition allows rapid, specific RNA
interrogation in cells. During my talk, I will present key examples: HuR scanning multiple RNA sequences, Hfq cycling RNAs, RuvC recognizing DNA Holliday junctions, and spontaneous RNA binding to HuR, SRSF1, Hrp48, and TbRGG2 RRMs. I will also mention the methodological challenges associated with computational research of biomolecular complexes.